Lestes rectangularis

April 25, 2025

Sample information
Picture
Photos by: Nour A.
Location
Collection date 11/12/2030
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The control placing is switched compared to the protocol. For clarification the gel image is correctly labeled with the controls and my arthropod is labeled N, Control labels: W- = negative, W+ = Positive, H= water (-) control, D= DNA + Wolbachia DNA (+) Control, LD= DNA ladders, the other’s are my groupmates.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level is low due to the fact that my observational results and my blast results did not match at all. My blast results stated that I extracted mite DNA from my arthropod  which can only occur if my arthropod was originally infected with a species of mite.  however from the observations and the photos you can clearly see that my insect is an odonata 100%.  Furthermore, once I had put the blast result on a phylogeny it showed that it was closely related to the German cockroach which is odd since mites are not insects and cockroaches are. Lastly, there was no wolbakia present in my DNA. As a result of the lack of consistency between results my confidence level and my research is low.

Wolbachia 16S sequence Download FASTA    Download AB1
GCGATCTTGGAGGGAATGCTGACCCCAGACATATTCCCGTGGGGTGCTGAGCCACAATAACTCTCGATTCCCCATTCTTG TGTTCTCGTTTCGCAGAGTATCCCAAAAGAGAGAGGTTTTTTAGGGATTACTTTACGCTTGCGCATCTTGCACCATTTTG TACACACCATTGTAGCACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGCTT ATCACTGGCAGTTTCCTTAAAGTACTCATTATTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAA CCCATCATCTCACGACACGAGATGACGACAGTCATGCAACACTTGTAAAAAATCCGGCCGAACCGACTCTATCCCTTCGA ATAGGTATGTTTGCGAGAAAATGCTATCTCGAGATCTACTCTATTAGAACGACTCATCTTCTTCCCAATCCCCCTATTAT AATACTAATTACTATAAAAAAAATTATAAACGAAGCATGAGCTGTATTATTACCTTATTTTTTGATCATCACTATTAACC TTCCTGGACGACCTATTTCTGACTTAATACACTTACTTATTATTTCTCTACCATTCCTGCTCCCCTACCAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GGGAAGAATGATTATATCGGATCTCATCCTCCTGCAGGATCAAAAAATGATGTATTAAAATTTCGGTCTGTAAGTAATAT TGTGATACCTCCTGCTAAAACTGGTAATGATAAAAAACAAAAAATAGTTGTCATGAAAATTGATCCAACGAATAAGGGGA TTTTGTCTGTTTTTATTGATGAAGGTTTTATTTTAATAGTTGAGGCTATAAAATTGATGCCCCCTAAAATTGAAAAAACT CCACCAAGGGGAAGGGAAAAAATTGCTAATTCTACGGAAGGGCCAGAGTGAGCGATATTTCTTGCTAATGGTGGGTAAAC TGTTCATCCAGTTCCAGCTCCTATTGATGTGAATGATCTGTAAAGACAAAGTGTGATTGATGGAGGCAATAGTCAAAATC TTATATTATTTATTCTTGGAAATGCTATGACGGGGGCTCTAAGTATTAAAGGAATTACCCAATTTCCGAATCCTCCAATT ATTATTGGTATTACTATAAATAAAATTATAATGAAGGGTGGGGATGTTATAATTGAGTTAAACATTTGCCCGATCAAAAT TAAACTTCCGGGTTGCCCAAATTCCAAAAAAATTAAGCTTCTAACGCTTGCTCCCTATATTCCTGAACATGCTCGAATAT AAAAACAATATCTTCATGTCTCGTATACATTGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Odonata was found to be negative for Wolbachia.
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