Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2024 students |
| Putative identification | Arthropoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The gel electrophoresis results (SS2A) had no bands and no usable DNA. The results may also be unreliable because the W- control had no results and the DNA control was positive for Wolbachia, which it should not have been, meaning the overall confidence level is low. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chauliognathus pensylvanicus was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid