Goldenrod soldier beetle

April 25, 2025

Sample information
Picture
Photos by: Sophia S., Cheryl H.
Location
Collection date 09/03/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The gel electrophoresis results (SS2A) had no bands and no usable DNA. The results may also be unreliable because the W- control had no results and the DNA control was positive for Wolbachia, which it should not have been, meaning the overall confidence level is low.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chauliognathus pensylvanicus was found to be negative for Wolbachia.
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