Scolia watanabei (wasp)

April 28, 2025

Sample information
Picture
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

2 wings, small, 6 legs, black and orange yellow coloring
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was no band in in the 438 region where Wolbachia normally is found. The identification was wrong according to the seek app. All of the controls worked great.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Hymenoptera was found to be negative for Wolbachia.
Report Inappropriate Post