Sample information
Picture	Photos by: Ibrahim A
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	collected by Fall 2024 students
Putative identification	Arthropoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	Low because small DNA concentration and thin band
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Chauliognathus pensylvanicus was found to be negative for Wolbachia.