Common eastern bumble bee

April 28, 2025

Sample information
Picture
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

Gathered around some wildflowers near a lake when it was 78F.
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus Bombus impatiens

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls showed up as expected on the gel so confident that the experiment worked.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Bombus impatiens was found to be negative for Wolbachia.
Report Inappropriate Post