Grass Skipper

April 28, 2025

Sample information
Picture
Photos by: Sara C.
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

Found in the fall in the wildflowers near lake Mayes, a terrestrial habitat at a temperature of 78ºF
Putative identification Arthropoda Insecta Lepidoptera Hesperiidae Atalopedes Atalopedes campestris

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: I made sure to crush the arthropod really well dissecting the abdomen and not the exoskeleton.

Gel Electrophoresis Lanes for CO1 Arthropod:

1- 1000bp ladder

2- Arthropod positive PCR Product

3- Wolbachia negative PCR Product

10- Control DNA

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All controls worked as expected. The arthropod band did appear on my gel as expected with no band appearing on the gel for Wolbachia. I had no problems with the protocol. So I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Atalopedes campestris was found to be postive for Wolbachia.
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