Western Honey Bee

April 28, 2025

Sample information
Picture
Photos by: Bethany C
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

The collection of samples took place in the fall when the weather was cooler. The terrestrial habitat (a lake) allowed for a good specimen collection. 
Putative identification Arthropoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked as expected and the BLAST database had a 100% sequence match for my arthropod. I did not have any difficulty throughout the process. Therefore I am confident my arthropod is not infected with Wolbachia. 
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Apis mellifera was found to be negative for Wolbachia.
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