Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Longwood University |
| Observations | |
| Putative identification | Arthropoda Insecta Odonata Coenagrionidae Enallagma Enallagma civile |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | The whole body (minus wings) was used and this caused the filter to get clogged, and did not allow the DNA to amplify. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The PCR did not amplify and there was no band during gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Enallagma civile was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid