Huron Sachem

April 28, 2025

Sample information
Picture
Photos by: Jay G.
Location
Collection date 10/31/2024
Captive / Cultivated? Wild-caught
Group Longwood University
Observations

Fall

78 Degrees Fahrenheit

Found in wildflowers near Lake Mayes
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Hesperiidae Atalopedes Atalopedes huron

Methods
Extraction kit promega wizard SV
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls worked as expected. There were no issues with the protocol, and none of the bands on the gel were surprising. The genetic identification of the organism matched the morphological identification of the organism, verifying its identity.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
ATTAGGAACTTCTTTAAGATTATTAATTCGAACAGAACTAGGTAATCCTGGATCTTTAATTGGAGATGATCAAATTTATAATACTATCGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTACCATTAATATTAGGAGCCCCTGATATAGCTTTCCCACGAATAAATAACATAAGATTTTGAATATTACCTCCTTCATTAACATTATTAATTTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCCTCAAACATTGCTCATCAAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCTGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACAACAATCATTAATATACGAATTAAAAATTTATCATTTGATCAAATACCTTTATTTGTATGATCTGTAGGAATTACAGCATTACTATTATTGTTATCTTTACCTGTATTAGCAGGAGCTATTACTATATTACTTACAGATCGAAATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Atalopedes huron was found to be negative for Wolbachia.
Report Inappropriate Post