ochlerotatus sticticus DS2

May 14, 2025

Sample information
Picture
Photos by: Benoit K.
Location
Collection date 03/19/2025
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

unknown
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Ochlerotatus Ochlerotatus sticticus

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Did most of the steps right maybe didn’t reduce the body enough therefore making a mistake in dna extraction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ochlerotatus sticticus was found to be negative for Wolbachia.
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