Myrmica Rubra DS1

May 14, 2025

Sample information
Picture
Photos by: Benoit K.
Location
Collection date 03/19/2025
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

unknown
Putative identification Arthropoda Insecta Hymenoptera Formicidae Myrmica Myrmica rubra

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Did every step carefull enough to not have done a mistake
Wolbachia 16S sequence Download FASTA    Download AB1
ATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAGGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGWW
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGAATAATTATTCGTKTAGAATTAGGATCTTGYAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATTCCTTTAATATTAGGATCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGTGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGGACCTTCAATTGATATATCAATCTTTTCTCTTCATATCGCTGGAATATCATCTATTTTAGGTGCTATTAATTTTATCTCAACAATTATTAATATACATCATAAATCAGTATCAATAGACAAAATCCCCTTAATAGTCTGATCAATTTTAATCACAGCTATTCTTCTCTTACTTTCCTTACCAGTCTTAGCAGGAGCAATTACTATATTATTAACAGATCGTAATTTAAATACTTCTTTTTTCGACCCATCTGGGGGGGGAGATCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCACCTGAAAAGTTWAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Myrmica rubra was found to be postive for Wolbachia.
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