Wolbachia-negative Harmonia axyridis

May 5, 2025

Sample information
Picture
Photos by: Zachary L.
Location
Collection date 04/01/2025
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Found inside of my house, recently deceased, surrounded by several others.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls utilized worked well, ensuring the authenticity of this test. The results are clear and have little obscurity, and the DNA sample sequences show no issues. As such, I believe it is reasonable to conclude that the test results are a true negative.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TTGGATATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACACCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCATTTACTATACTATTAACTGAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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