Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Neuqua Valley High School |
| Observations | The cedar beetle was found in an old foundation of a shed that houses broken and rotted wood. The beetle was relatively inactive with slow movements throughout the wreckage. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | The band was really faint and there wasn’t enough DNA to get a sequence. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | There wasn’t enough DNA to actually come to a full conclusion, so our confidence level is low. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman