NV 67 Cedar Beetle

April 15, 2021

Sample information
Picture
Photos by: Daniel C., Aarav S.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The cedar beetle was found in an old foundation of a shed that houses broken and rotted wood. The beetle was relatively inactive with slow movements throughout the wreckage. 
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

The band was really faint and there wasn’t enough DNA to get a sequence.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There wasn’t enough DNA to actually come to a full conclusion, so our confidence level is low.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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