NV 48 Pill Bug

April 15, 2021

Sample information
Picture
Photos by: Vishnu S.
Location
Collection date 10/04/2020
Captive / Cultivated? Captive / Cultivated
Group Neuqua Valley High School
Observations

It was under a plant pot. I looked under the plant pot and there was this pill bug. There were only two there. There was only mud around it. There was no greenery from where I could see. It was on a wooden deck in which it was moist.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download AB1
Arthropod COI sequence
Summary The Isopoda was found to be postive for Wolbachia.
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