Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/04/2020 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Neuqua Valley High School |
| Observations | It was under a plant pot. I looked under the plant pot and there was this pill bug. There were only two there. There was only mud around it. There was no greenery from where I could see. It was on a wooden deck in which it was moist. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Puregene (Qiagen) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | LB |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Isopoda was found to be postive for Wolbachia. |
Water Boatman