Sample information
Picture	Photos by: Christine H.
Location
Collection date	10/02/2024
Captive / Cultivated?	Wild-caught
Group	PMS Kreuzlingen
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Chironomidae Tanytarsus Tanytarsus pallidicornis
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence	Download FASTA    Download AB1
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Tanytarsus pallidicornis was found to be negative for Wolbachia.