Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Pholcidae Pholcus Pholcus phalangioides |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Body without legs |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | 1. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp) 2. DNA sample of Pholcus phalangioides 3. DNA sample of Pholcus phalangioides 4. DNA sample of Armadillidium vulgare 5. Duplex positive control 6. Duplex negative control |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The +Dna control worked, but the +Wolbachia Control did not work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Pholcus phalangioides was found to be postive for Wolbachia. |
Baetis_C2.2
Formica_C11
Philaenus sp._C8
Ichneumonidae_A12
Formica_A11