Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Unionville High School |
| Observations | |
| Putative identification | Arthropoda Insecta Diptera Chironomidae Chironomus Chironomus tentans |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Repeated trials with other Chironomus Tentans revealed positive results too. Months of trials were spent refining our experimental process in order to yield trustworthy results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chironomus tentans was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
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Wolbachia data
Meadow Katydid