Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/30/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | Fast Blast |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Based on our gel, there were two clear bands which indicates that Wolbachia is present in the arthropod. Our controls worked which leads us to be confident in trusting our results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
NNNNNNNNNNNNNNNNNNNNTNNNNNNNNNGNNTGNCGNNTCNCNNNGAANNNGNNNNNNNNNGGNNANNNNGNNNCCNN NNNNNNTTNNNNNCATCCTNNTCTTACCNAATTGCTCACGGNGGNAGAATGNCCCCGTCACTGCCCCTGTAAGAGTGTNG GNGGNNGGNGNTCAAATATNATCGGGNGTTGGGGGTGGGTGATNGACNNCATNCATTNGAGGCTATNANATANTCTTTTN NTTNACTAACTAAAGTAANAANNTATCTTANTACCCCNNCTTTATTTTTTATTGNTTACNANNAANTTGGTGTGAANNNN ATTAATANCNAGANGNNTNNTTNTTTTTTTTGCTGGTNTTTTNTTCNTTTTTTTTTTTTTTANCNNNNNNCNNNNGNNNN NNNNNTNNNNNTTANNNTANNNATNNANNNTTTNNTNNNN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Coleoptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid