Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | 5. PCR molecular weight ruler (1000, 700, 500, 200, 100 bp) 6. Water 7. Positive DNA Control 8. DNA sample of Armadillidium vulgare |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No band was present in the lane, which indicates no presence of Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidium vulgare was found to be negative for Wolbachia. |
Baetis_C2.2
Formica_C11
Philaenus sp._C8
Ichneumonidae_A12
Formica_A11