Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/09/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Insect was found under a discarded piece of wood. |
| Putative identification | Arthropoda Hexapoda Insecta Neuroptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Larva |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | 100X Fast Blast |
| Gel images |
|
| Protocol notes | While a gel was run with Plodia Interpunctella samples/DNA gel electrophoresis was not run properly/ samples were not prepared correctly so no bands appeared on the gel. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | No samples were submitted to be barcoded so it is unknown whether the Plodia Interpunctella contained Wolbachia. Additionally, during gel electrophoresis, no bands were seen for this sample including the wolbachia positive band and any other band proving PCR/DNA extraction was unsuccessful. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Baetis21Cb2.1
Diptera21Aa2.1
Baetis21Aa6
Baetis21Aa2.3
Diptera_Cb6