GRP9 Dysdera Crocata

May 26, 2025

Sample information
Picture
Photos by: Brayden N.
Location
Collection date 04/30/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

This arthropod had a very large abdomen compared to our other samples.
Putative identification Arthropoda Chelicerata Arachnida Araneae Dysderidae Dysdera Dysdera crocata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Gel 1
Lane
1 – PCR MW Ladder (failed)
2 – centipede (lamyctes emarginatus)
3 – pillbug (armadillidium vulgare)
4 – woodlouse spider (dysdera crocata)
5 – ground beetle (anisodactylus binotatus)
6 – Arthropod Positive Control
7 – Arthropod Negative Control
8 – Drosophila Positive Control
9 – Drosophila Negative Control

Gel 2
Lane
3 – PCR MW Ruler
6 – MiniOne Ladder

Our first gel’s ladder did not run successfully, so we ran another gel with the same ladder and and another MiniOne ladder and compared the relative mobilities of the two gels to determine the base pairs of the first gel.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Although our controls worked,  we are unsure of why our ladder did not run, so the results of our gel are somewhat questionable. However, all of the samples ran correctly, with aligned bands that were only in expected locations.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNNNNNNNNTNGNTNGCTTGGTCTGCTATAGTGGGTACAGCAATAAGAGTCTTAATTCGGGCGGAGTTGGGTCA GGTTGGAAGATTATTGGGTGATGATCATTTGTTTAATGTGGTGGTTACAGCGCATGCTTTAGTTATGATTTTTTTTATAG TAATACCTATTATAATTGGAGGATTTGGTAATTGGTTAGTGCCTTTGATGCTTGGAGCTCCTGATATGGCTTTTCCTCGT ATGAATAATTTAAGTTTTTGGTTGTTGCCTCCTTCTTTAATTTTATTAGTTATTTCTTCGATAGTGGAAATAGGGGTGGG GGCTGGGTGAACGATTTATCCCCCCTTGTCAGGGGCATTAGGGCATGCCGGAGTGTCGGTAGATTTAGCTATTTTTAGCT TGCATTTAGCTGGGGCTTCTTCTATTATGGGGGCAATTAATTTTATTTCTACAATTTTGAATATGCGGTCTGAGGGAATA TCTTTGGATAAGGTACCTTTGTTTGTGTGATCTGTTTTGGTAACGGCTGTTTTGTTATTGTTGTCGTTGCCAGTTTTAGC TGGGGCGATTACTATGCTATTAACGGATCGGAATTTTAATACATCTTTTTTTGACCCAGCGGGAGGAGGGGATCCTATTT TGTTTCAACATTTATTTTGATTTTTTGGTCACCNGNNAAGTTNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dysdera crocata was found to be negative for Wolbachia.
Report Inappropriate Post