Sample information

This arthropod was taken from the campus of CAMS Highschool, which was then stored in a Petri dish until performing the following procedures. Black colored exoskeleton. Segmented body, about 1.5 cm long. Found under benches in grass.

Methods

Lab2_DNAExtraction_Mar2022

All lanes not labeled do not contain anything in them.
Lane 1 Contains the PCR Molecular Weight Ruler.
Lane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
Lane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
Lane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
Lane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
Lane 6 Contains the Infected drosophila melanogaster Positive Control
Lane 7 Contains the Uninfected drosophila melanogaster Negative Control
Lane 8 Contains the positive DNA Control
Lane 9 Contains Nuclease Free Water

This specimen’s bands can be located on the labeled lane 4.

Results

Due to a possible error when performing DNA extraction, no results were visible in our gel after electrophoresis, except for the provided controls. Thus, an unsuccessful lab was performed. Out of the four Armadillidium Vulgare samples, only one had a band indicating presence of Arthropod CO1 gene, and none had any bands indicating presence of Wolbachia 16S rRNA gene.