Armadillidium Vulgare (H2 Garden sample Lane 3) GRP 1

May 28, 2025

Sample information
Picture
Photos by: Melanie H., Keiry M., KeJuan J., Celine J., Callisto R.
Location
Collection date 05/13/2025
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

This arthropod was collected from a pot in a garden. Black colored exoskeleton with yellow designs in the center of its body. Segmented body, about 1.75 cm long.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

Lab2_DNAExtraction_Mar2022

All lanes not labeled do not contain anything in them.
Lane 1 Contains the PCR Molecular Weight Ruler.
Lane 2 Contains the Armadillidium vulgare (H1 Garden Sample)
Lane 3 Contains the Armadillidium vulgare (H2 Garden Sample)
Lane 4 Contains the Armadillidium vulgare (S1 School Campus Sample)
Lane 5 Contains the Armadillidium vulgare (S2 School Campus Sample)
Lane 6 Contains the Infected drosophila melanogaster Positive Control
Lane 7 Contains the Uninfected drosophila melanogaster Negative Control
Lane 8 Contains the positive DNA Control
Lane 9 Contains Nuclease Free Water

This specimen’s bands can be located on the labeled lane 3.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Due to a possible error when performing DNA extraction, no results were visible in our gel after electrophoresis, except for the provided controls. Thus, an unsuccessful lab was performed. Out of the four Armadillidium Vulgare samples, only one had a band indicating presence of Arthropod CO1 gene, and none had any bands indicating presence of Wolbachia 16S rRNA gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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