Melanoliarus placitus

June 5, 2025

Sample information
Picture
Photos by: Liam Scoon
Location
Collection date 05/30/2025
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

The bug was found in the forest part of Pingry School, under a big rock. The bug moved very slowly when the rock was lifted. It had a white body color, 2 eyes, and was smaller than a grain of rice.
Putative identification Arthropoda Insecta Hemiptera Cixiidae Melanoliarus Melanoliarus placitus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

First, DNA was extracted from the bug, then purified the DNA, and then eluted the DNA. After starting PCR. After PCR was created and loaded the gel was loaded. After we did PCR purification and then sequencing.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my bug is not Wolbachia positive because when looking at the gel, my Wolbachia sample matched my negative control, showing that it is not Wolbachia positive.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNGACTTATNNGNNNNATNNNANNAATAATTATCCGAATAGAATTAACTCAT CCNGGCTCAATCATCAACAACGATCAAATCCACAACATAATANTAACCTCACATGCTTTCATTATGATCTTCTTTATAGT NATACCAATTATAATTGGAGGATTCGGAAACTGATTAGTCCCAATTATAATTGGAGCTCCNGACATAGCATTCCCCCTAA TAAACAACATAAGATTTTGACTCCTACCACCTTCTTTAATATTAATAATTTCCAGAAGATTAACAGGATCTGGAGCAGGG ANNGGGNGAACAGTATATCCACCTCTGGCTAGATTAACAGCCCATTCAGGCCCATCTGTAGAGATNACAATTTTTTCTCT CCCTATTGNANGTGTTAAATCAATCCTAGGAGNAATCTACTTTATCTCNACTATTTTAAATATACGATCANAAGAAATAA CACCNGAAAAAACACCCCTATTTTGTTGATAAGTTCTAATTACAGCNATTTTNCTATTAATTTCNCTNCCCNTGTNNTAG GAGCNATTANTGTATTACTTATNGACCGAAATTTTCATANAACATTCTTTGATCCTACCTGAGGAGGAGATCNACTNNTA NTTANCAATTATTCTGATGATTTGNTNNACNGNNTNNAANTAGN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoliarus placitus was found to be negative for Wolbachia.
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