Hydrophili

June 6, 2025

Sample information
Picture
Photos by: Ishaan K.
Location
Collection date 05/30/2025
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

I found the bug next to the birdfeeder at the Pingry School. It was in a patch of dirt, and there were numerous specimens of the same species. The arthropod has a smooth, oval body and is black. It is around 2 cm in length and 0.5 cm in width.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The first step was DNA extraction, then DNA purification, and then DNA elution. After that, I started PCR and created my gel. During my gel loading, I accidentally put my Wolbachia sample on the top row. It is the last well in the top row and is showing DNA at that well, signifying that there is Wolbachia present in my arthropod sample. After this, I did PCR purification and finally sequencing.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident that my bug has Wolbachia because in the gel, there is DNA present in the Wolbachia sample well and matches the positive control well. Also, in my sequencing of the Wolbachia sample, I have a 99.31% match with “uncontrolled Wolbachia”. This makes me confident in my findings.
Wolbachia 16S sequence Download FASTA   
NNNNNNNGNNGNNNNNNNNGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCN CAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGG ATGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
NNNNNNNNNNNNNNNNNNNNNNNNGTTNANATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGN NNNACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATAC CTTTTATAATTGGGGGATTTGGTAATTGATTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAAT AACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGG TTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCNNNNNGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATA TTGCTGGNATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACNATTATAAATATACATAATTCCAATATTTCCCNN NATAAAATTCCATTATTATNATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGGGC CTATTNNNTACTACTAACAGACCGAAAATCTTAATACTTCATTTTTTCGATCCCTCGGGGAGGANGAGATCCTATTTTTA TACCAACATTTATTTTGAATTTTTTGGGTCACCCTTGAAAGTTTAAN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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