Sample information
Picture
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Neuqua Valley High School
Observations
Putative identification	Arthropoda Insecta Diptera
Methods
Extraction kit	Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system	Standard electrophoresis system
Buffer	LB
DNA stain	GelRed
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be negative for Wolbachia.