dobson fly

September 11, 2023

Sample information
Picture
Photos by: Juveria
Location
Collection date
Captive / Cultivated? Captive / Cultivated
Group Neuqua Valley High School
Observations

I got the bug outside my front door by the light on the top.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: I mase sure to crush the arthropod really well. I dissected the abdomen out and didn’t include the exoskeleton.

Gel electrophoresis lanes for CO1arthropod PCR:

1- 1 Kb ladder

2- The second well is my negative DNA extraction control.

3- The third well is the positive DNA extraction control

4- the fourth well is positive PCR/DNA control

5-the fifth well is positive PCR control

6- the sixth well is my sample

Analysis: my controls worked but my sample did not have a band at all.There could have been a problem with my extraction

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected.  I did not have any problems with the protocol, and i was not surprised by any bands on the gel. Therefore I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
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