Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Neuqua Valley High School |
| Observations | I got the bug outside my front door by the light on the top. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA extraction: I mase sure to crush the arthropod really well. I dissected the abdomen out and didn’t include the exoskeleton. Gel electrophoresis lanes for CO1arthropod PCR: 1- 1 Kb ladder 2- The second well is my negative DNA extraction control. 3- The third well is the positive DNA extraction control 4- the fourth well is positive PCR/DNA control 5-the fifth well is positive PCR control 6- the sixth well is my sample Analysis: my controls worked but my sample did not have a band at all.There could have been a problem with my extraction |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of my controls worked as expected. I did not have any problems with the protocol, and i was not surprised by any bands on the gel. Therefore I am confident my arthropod is not infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidae was found to be negative for Wolbachia. |
Water Boatman