Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | The spider was found under a leaf, balled up. |
| Putative identification | |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There could have been an error in extracting the DNA from the initial specimen. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
NNNNNNNNNNNNNNNNTGNNCTGCTATATTAGGAACAGCAATAAGAGTATTAATTCGAATTGAGTTAGGTCAACCGGGTA GATTTATAGGTGATGACCAATTATATAATGTAATTGTAACTGCTCATGCATTTGTAATAATTTTTTTTATGGTTATGCCA ATTTTAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTTCCACGAATGAATAA TTTAAGATTTTGATTATTACCTCCTTCATTATTATTACTAATTACTTCATCAATAGTTGAAATAGGGGTTGGTGCTGGTT GAACAGTATATCCTCCTTTGGCTGGATTAGAAGGACATGCTGGTAGCTCAGTTGATTTTGCTATTTTCTCTTTACATTTA GCTGGGGCTTCTTCAATTATAGGGGCAATTAATTTTATTTCTACTATTATTAATATACGTTTTTATGGAATGACTATAGA AAAAGTTCCTTTATTTGTATGATCTGTATTAATTACAGCTGTTCTATTATTATTATCTTTACCAGTATTAGCTGGGGCTA TTACAATATTATTAACTGATCGAAATTTTAATACTTCTTTTTTTGATCCTTCTGGGGGTGGAGATCCTATTTTATTTCAN CATTTGTTTTGATTTTTTGGTCACCTNNAAANNTNNN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid