Eurydema gebleri voucher – Lady Beetle

September 11, 2025

Sample information
Picture
Photos by: Alexis Trella
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Red, black, circular, small, spotted.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 9- LTHZ 01 – DNA standard

Lane 10- LTHZ 01 – Arthropod 1 WOL + COI1 (Grasshopper)

Lane 11- LTHZ 01 – Arthropod 2 WOL + COI2 (Ladybug)

Lane 12- LTHZ 01 – WOL + (Positive)

Lane 13 LTHZ 01- WOL – (Negative)

Lane 14- LTHZ 01 – gDNA CNTRL

Lane 15- LTHZ 01 – Water

Lane 16- LTHZ 01 – Empty

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am very confident in my results as the 2 bands that appeared were very vivid on the gel. With other research done as well, I know many ladybugs especially males are known to be positive.

 
Wolbachia 16S sequence
NNNNNNNNNNGNNNNNNNGNGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGC AACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGA TGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCG AGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGT AATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGNTNCN NNT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ANNNNNNNTTNNNNNNNNGNANNNGGGCAGGAATAGTAGGAACATCGTTNNNTATTTTAATTCGGTTAGAATTANNANNN NNAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGT AATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGAT TAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGA ACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTT ACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAA TACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCA GGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTT ATACCAACATTTATTTTGATTTTTTGGTCACCTNGNAAGTTNAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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