Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Fuzzy greenish white color, black whisker like antennas |
| Putative identification | Arthropoda Insecta Lepidoptera Erebidae Halysidota Halysidota tessellaris |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | |
| Gel images |
|
| Protocol notes |
|
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | No Wolbachia was present in some of the Arthropods but present in others. Wolbachia only present in the Arthropod in lane 2. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGNATTTNAGCAGGTATAGTAGGAACTTCTTTAAGATTATTAATTCGAGCTGAATTAGGAAACCCTGGCT CTTTAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTAT AGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATA GCATTTCCTCGAATAAATAATATAAGTTTTTGACTTTTACCCCCATCATTAACTTTATTAATTTCAAGAA GAATTGTAGAAAATGGAGCCGGAACAGGATGAACAGTTTATCCACCCCTTTCATCTAATATTGCCCATGG AGGTAGATCAGTAGATTTAGCTATTTTTTCCCTTCATTTGGCAGGTATTTCTTCAATTTTAGGAGCAATT AATTTTATTACTACAATTATTAATATACGATTAAATAACTTATCTTTTGATCAAATACCTTTATTTGTTT GAGCTGTAGGAATTACAGCATTTCTATTATTACTTTCATTACCTGTATTAGCAGGAGCTATTACAATATT ACTAACTGATCGAAATTTAAATACATCATTTTTCGACCCTGCTGGAGGAGGAGATCCTATTCTTTACCAA CATTTATTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Halysidota tessellaris was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid