Flatormenis proxima – Leafhopper

September 11, 2025

Sample information
Picture
Photos by: Hatice E.
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

It is small and it has a color like light green to white. It has a big wings for its body. It looks like a type of butterfly. The size of the fragment was approximately a few mm in length. They are not harmful to the people, but since they eating the plants, the plant can be weak because of this creature.
Putative identification Arthropoda Insecta Hemiptera Flatidae Siphanta Siphanta acuta

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

We followed the standard Wolbachia Project protocol to extract DNA and run PCR. After setting up the reactions, we ran the samples on an agarose gel. It was interesting to see how DNA bands appeared under blue light, showing whether our arthropod was infected or not.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence level is medium since I was little nervous. I was not sure how I was looking my gel results, but once I start understanding the process got better. I was the only one in my table who has positive Wolbachia. The results seems reliable.
Wolbachia 16S sequence
GGNGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGCACTTTAAGGAAACTGCCAGTGATAAGTTGGAGGAAGATGGGGATG ATGTCAAGTCATCATGGCCTTTATGAAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAA GCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAA TCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ATCNGGACTAGTAGGATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCA ATAATCAAAAATGATCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAAT TATAATTGGTGGTTTTGGAAACTGACTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATA TAAGTTTTTGACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGA ACTGTATACCCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGATTTAACAATTTTTTCATTACACATTGC AGGTATTAGATCAATTATAGGAGCAATTAATTTTATTTCGACCATTATAAATATGCGAATCAAGGGAATAACAATAGAAA AAACCCCCTTATTTTGTTGATCAGTACTAATTACAGCAATCCTTTTACTAGTTTCTTTACCAGTACTAGCCGGAGCAATC ACAATATTAATTATAGACCGAAATCTAAATACTTCATTTTTTGATCCATCAGGAGGAGGCGATCCTATTCTGTATCAGCA CTTATTCTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Siphanta acuta was found to be postive for Wolbachia.
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