Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Caught with net in a larger forest area. It was in a bush. Anthropod is green, roughly inch in length. One antenna is visibly broken off. When collected, grasshopper made loud buzzing noise. |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | Cyber Stain |
| Gel images |
|
| Protocol notes | Lane 1: SGGS03W1 -> Grasshopper Lane 2: SGGS03W2 -> Stinkbug Lane 3: SGGS03W3 -> Positive Control Lane 4: SGGS03W4 -> Negative Control Lane 5: SGGS03W5 -> DNA Control Lane 6: SGGS03W6 -> Water |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No bands shown |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica