Bombus impatiens (Common eastern Bumble Bee)

September 11, 2025

Sample information
Picture
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The researchers collected the arthropods from outside then followed the wolbachia protocol listed on the website.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The researchers forgot to load the DNA ladder resulting in unclear results.

 
Wolbachia 16S sequence
ATCGNNNTNNATGAANAGTAGAGTTGAGNGNGAATNANNTNNCCAGGA TTTTGGGCNCTGNNATTATTGTNNATTGTGTGTCNNTTTACGCTNNTTTTATTTNTTTNNTTNNNNATANNANNATTNNA NTGGAGGANGNAGAANATGATTNANCTNTAAAGTTGAGGACCCCCNTAATTATNATNCCCCCNAANANTNTTTAAATTTT ATCCCNTCCCCCTCCATCTCNTTATTATTGTATCTTAANNTTTNNTCTGGNGTNNNGNGACCATGAGTACTNCTNTCCCC CTNTTTCNNTTTATTTCTNNNTGGATCGGCNNCGGTATCTTTANTTTTTTTGTTTTTACATNNANCNGGAATTCTTNCTN TNGNATCTNTCAACTGTATTAATTATANTATNAACAGGCNAACGGTAGTTCGNNNNCTGATCCCATTCCATCATTTGATC GAGCTGAANGATTTNCATNATTTTCATTATTATTATCNNNACCGGTANNANNNGATTATTNNNNGATCTAATATNCNGAC CTCAATNTATNACCNNCTTATNNNGNCCCNNCTGGNGAGGNNNNNNTATCTNAATCNNNNNAATTNTGATCTTNGNCNNN NN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
ATGATNNGGATAATCGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAAT ATGAATTAATAATGATCAAATTTATAATTCATTAGTTACAAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCAT TTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATAAT ATTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTG AACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATGTAA CAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTTGCTATTATATTAATAAAAAATTTTTCATTAAATTATGAT CAAATTNCTTTATTTTCATGATCTGTATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGCAGGAGCAAT TACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAAC ATTTATTTTGATTTTTTGNC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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