Schinia Trafica / Moth

September 11, 2025

Sample information
Picture
Photos by: Ahmed S
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Cyber Stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

We forgot to add ladder
Wolbachia 16S sequence
GTCTGGTATCCTCTCAAAANTGAGATNNCGAGNAAAATCCNCNACCCT GGANCCNTNNTNNGGNGACATTTTTANTNNCTGTTGACTCTNCNCACGNGTTTTTAATANNTTTNTNAAAGGTNGGNATT ATGTNNTGCATGNGNNNCNANATGGANAGCCTACATCNTGAGGAGNGNTGGAAANNTGTGCCNCNAATANNNNATGAAAT NTNNGCCCTTNNNCCCATCTTAATTTTTNTTGTGCTCNNCAACANNTTTGAAAAAAGTAGTGNTACCAGGAAAACTGGAA TNCCCCCCTCATGNTNAATACTTNNNCGTGTCNGGACCTCNNTGCCCGTAACTCNTTTNTAATTGGATNTAGCAGGAANT NNTNCTATTTTAGGAGCTTTTAATTTTNTTACAACAATTATTAATATACGATTAAAAAGTTTACCTTTTGATCNATACCA TTATTTTNNGAGCAGAAGGAATTACAGCATTTTNTTTATTATTATCTTTACCTGTATTAGCTGGAGCTATTACAATATAA TAAACAGATCTAAATTTAAAAACTTCTTTTTTTTGACCCAGCTNNNNNANGGANNNCNNATNTTANTCNNNTTAATTNTG ATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GCTGGTATAGTAGGAACTTCTCTAAGATTATTAATTCGAGCAGAATTAGGGAATCCTGGATCT TTAATTGGAGATGATCAAATTTATAATACTATTGTGACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATACCTAT TATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGAGCCCCAGATATAGCTTTCCCCCGAATAAATAATA TAAGTTTCTGACTTTTACCCCCATCTTTAACATTATTAATCTCTAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGA ACAGTTTACCCCCCACTTTCATCTAATATTGCCCATGGAGGTAGTTCTGTAGATTTAGCTATTTTTTCTTTACATTTAGC AGGAATTTCTTCTATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGATTAAATAATTTATCTTTTGATC AAATACCATTATTTATTTGAGCAGTAGGTATTACAGCATTTTTATTATTATTATCTTTACCTGTATTAGCTGGAGCTATT ACAATATTATTAACTGATCGAAACTTAAATACATCTTTTTTTGACCCAGCTGGTGGGGGAGACCCTATTCTTTATCAACA TTTATTTTGANTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be postive for Wolbachia.
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