Green cone-headed planthopper (Acanalonia conica)

September 11, 2025

Sample information
Picture
Photos by: Shawn T.
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

 
Putative identification Arthropoda Insecta Hemiptera Acanaloniidae Acanalonia Acanalonia conica

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

  • There was only one yellow bar in the column for the arthropod, when a wolbachia positive would have a second
Wolbachia 16S sequence
Arthropod COI sequence
NNNNNNNNNNNNNNNGGTTATGATNNGGAATAANTGGGACANTANTAAGAATAATTATTCGTATTGAATTANCTCAGCCA GGATCANTNATTAAAAATGACCAAATTTATAATTNNATTGTTACNNNACACGCATTTATTATAATCTTTTTTATAGTTAT ACCAATTATAATGGGAGGATGGGGAAACTGACTAGTTCCNATAATAATTGGAGCCCCANATATAGCATTTCCACGAATAA ATAATATAAGATTTTGACNANNACCAATATCCCTATCAATATTAATTATAAGATCAATTTCAGGTTCAGGAGTTGGAACA GGATGAACAGTTTACCCTCCANTATCTAGTCAAANTGCCCATTCAGGACCATCAGTAGATATAGCAATTTTTTCACTTCA TATTGCAGGTATTANNTCAATTATGNNNNNNATTAACTTTATCTCAACAATTTTAAATATACAAACAAAGGGAATAACTA TAGAAAAAACACCACTTTTTTGTTGATCAGTTCTTATTACAGCAGTTTTANNNNTAGGTTCATTACCAGTNTNAGCCGGA GCAATTACAATATTATTAATAGATCGAAACTTCAANACATCATTTTTTGACCCATCAGGAGGGGGGAGATCCAATTCTNT ATCANCATTTATTTTGATTTTTNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Acanalonia conica was found to be negative for Wolbachia.
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