Sample information
Picture	Photos by: Mason A
Location
Collection date	09/11/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations
Putative identification	Arthropoda Insecta Lepidoptera Crambidae Agriphila Agriphila straminella
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Green
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	There was only one yellow bar, a specimen that is wolbachia positive would have two yellow bars
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Agriphila straminella was found to be negative for Wolbachia.