Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/09/2025 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Galileo Academy of Science and Technology |
| Observations | -about 2cm mosquito, has long legs and antennas, female mosquito |
| Putative identification | Arthropoda Insecta Diptera Culicidae Culex Culex pipiens |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | There is no indication of a second line under the first that will signal in gel electrophoresis that Wolbachia would be present. However, there might be a risk of contamination, as our negative control shows results when it’s not supposed to. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex pipiens was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica