Sample information

The moth was still for over 24 hours on my outdoor screen door. thinking it was dead. I put it into my tube and it began to fly around aggressively

Methods

We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product # T3010)

The specimen was incubated for 30 minutes in a hot water bath at 56 degrees C.

Our second Gel image was taken on 11/4/25 and was run at 125 volts for 25 minutes

used this DNA ladder: New Englands Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)

On our second PCR reaction was set up on 10/28/25, used the same Taq polymerase as the first PCR reaction, and:

was a single reaction that used the Arthropod primers

used an annealing temperature of 49 degrees Celsius.

Results

I am confident that my experimental results were valid. We followed protocol exactly and were very cautious about cross contamination. The bands look as they should- clear, bright, and the accurate size.

I am still confident that my results were valid. We used Arthropod CO1 primers for our single PCR and Wolbachia bands were clearly not present the first time we did Gel Electrophoresis and followed protocol exactly.