KP Megalopyge Opercularis 09-16-25

December 15, 2025

Sample information
Picture
Photos by: Khush P
Location
Collection date 09/15/2025
Captive / Cultivated? Wild-caught
Group Chelmsford High School
Observations

Small pond bordered by tall green grasses and vegetation, and grass around.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

20 minutes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My data was clear and I am pretty confident..
Wolbachia 16S sequence Download AB1
Arthropod COI sequence
Summary The Lepidoptera was found to be postive for Wolbachia.
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