Sample information
Picture	Photos by: Khush P
Location
Collection date	09/15/2025
Captive / Cultivated?	Wild-caught
Group	Chelmsford High School
Observations	Small pond bordered by tall green grasses and vegetation
Putative identification	Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	20 minutes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	I am sure my data is correct.
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence
Summary	The Melanoplus femurrubrum was found to be negative for Wolbachia.