Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Chelmsford High School |
| Observations | The insect was caught at Chelmsford High School near a small pond called the science pond. The area is grassy and has abundant vegetation. The grasshopper was found in a bed of dirt and near what used to be a garden area. |
| Putative identification | Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus punctulatus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Loaded 10 microliters and ran for 20 minutes |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | My negative arthropod control showed results as if it were a positive arthropod control which lessens my confidence |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Melanoplus punctulatus was found to be negative for Wolbachia. |
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