Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/15/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Chelmsford High School |
| Observations | Caught at Chelmsford High School near a small pond known as the science pond. The area is grassy and abundant vegetation as well as rock areas. Ant was found on rocks. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium caespitum |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | 10 microliter load ran for 20 minutes |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Data came back with positive BLAST hits for Wolbachia |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Tetramorium caespitum was found to be postive for Wolbachia. |
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