Sample information

Brown with black spots and red spot beneath wings. 2 wings. 6 legs. Oval abdomen. No visible antennae.

Captured on a concrete pool deck.

Late summer

Temperature: 75º

Habitat: urban

Methods

We added 10 extra microliters or Proteinase K during DNA extraction. We accidentally filled two wells with DNA in the CO1 gel, but no contamination.

Gel electrophoresis, Arthropod CO1: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, C-6 in lane 4 (accidentally split C-6 into two lanes, but both showed the same results and there was no contamination.) W+ in lane 5, W- in lane 6, H2O control in lane 7. Controls worked.

Gel electrophoresis, Wolbachia 16S: DNA ladder in lane 1, C-5 in lane 2, C-6 in lane 3, W+ in lane 4, W- in lane 5, H2O control in lane 6. No mistakes. Controls worked.

The primer did not bind correctly for DNA sequencing, the quality scores were not high enough to blast. However, we are able to tell that it is a Spotted Lantern Fly because of its morphology and local knowledge.

Results

All the controls worked and the lines were strong, but during DNA sequencing, the primer for the CO1 did not correctly bind, and despite rerunning it with a more specialized primer, we were unable to precisely identify it down to species because the quality was so poor. However, we are certain that it is a Spotted Lanternfly as they are invasive and well known in our area.