Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | |
| Putative identification | |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | 1-8, Common hentz jumping spider (first line) Praying mantis (second line) Wolbachia present in spider. 9-16: first line: European harvestman spider second line: Tussock moth caterpillar No Wolbachia present |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Very sure of wolbachia presence on first organism in lane 1 for the spider. No wolbachia presence for the mantios |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid