Philoscia muscorum

October 16, 2025

Sample information
Picture
Photos by: Elena V
Location
Collection date 09/20/2025
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations
  • Brownish color
  • Terrestrial area w/ lots of leaves & dirt
  • Caught summer going into fall
  • 73 degrees when caught
  • Very slim
  • 8+ legs
  • Antennaes
Putative identification Arthropoda Malacostraca Isopoda Philosciidae Philoscia Philoscia muscorum

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction

  • The arthropod crushed up easily

PCR

  • Everything went as planned

Gel Electrophoresis, Arthropod CO1

Image 1:

  • Lane 1 = DNA LADDER
  • Lane 2 = A-1
  • Lane 3 = A-2
  • Lane 4 = A-3
  • Lane 5 = A-4
  • Lane 6 = Wolbachia (+)
  • Lane 7 = Wolbachia (-)
  • Lane 8 = H20

Image 2:

  • Lane 1 = DNA LADDER
  • Lane 2 = A-5
  • Lane 3 = A-6
  • Lane 4 = Wolbachia (+)
  • Lane 5 = Wolbachia (-)
  • Lane 6 = H20

Gel Electrophoresis, Wolbachia

  • Everything went as planned, our controls worked

 

  • Lane 1 = DNA LADDER
  • Lane 2 = A-1
  • Lane 3 = A-2
  • Lane 4 = A-3
  • Lane 5 = A-4
  • Lane 6 = Wolbachia (+)
  • Lane 7 = Wolbachia (-)
  • Lane 8 = H20

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high because our controls worked!
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Philoscia muscorum was found to be negative for Wolbachia.
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