Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | |
| Putative identification | Arthropoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | ] |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All positive and negative controls worked, and there was no band for my sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGCACTAAGAACATTAATTCGAATTGAATTGGGAATTCCTGGGTCATTCATTGGTGATGATCAAATTTATAATGTTGTTG TTACAGCTCATGCATTTATTATAATTTTTTTCATGGTTATACCTATTATAATAGGAGGATTTGGTAATTGACTTGTACCA TTGATAATTGGGGCACCTGATATGGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTGCCCCCTTCTTTGACCTT ATTATTAGTTGGAAGATTAGTTGATAGAGGTGCTGGAACTGGTTGAACAGTTTATCCTCCTTTATCAAGATACATATTTC ATTCTGGTTCTTGTGTTGATTTAACAATTTTTTCTTTACATTTGGCAGGTGTATCTTCAATTTTGGGGG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Insecta was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica