Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Found under the eaves of a porch. Damaged horn, couldn’t walk, seemed like it was dying. |
| Putative identification | Arthropoda Insecta Coleoptera Scarabaeidae Dynastes Dynastes tityus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls worked, and there was no band for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TCACTAAGTCTCCTAATTCGAGCCGAACTAGGAAACCCTGGGTCATTAATTGGTGATGATCAAATCTATAATGTTATCGTAACAGCCCACGCTTTTATTATAATTTTTTTTATAGTTATACCCATTATAATTGGGGGATTCGGAAATTGGCTTGTCCCCCTAATACTTGGGGCCCCGGATATAGCCTTCCCACGAATAAATAATATAAGATTCTGGCTCCTCCCACCCTCATTAACCCTTCTTCTAGCCAGAAGAATAGTAGAAAACGGGGCCGGAACTGGCTGAACAGTATACCCTCCCCTCTCTGGGAACATTGCTCNTAGAGGAGCCTCTGTTGATCTCGCAATTTTTAGGCTACACTTAGCAGGANTTTCTTCTATTCTAGGTGCAGTAAATTTTATTACGACTGTAATTAATATNCGTTCAACCGGAATATCTTTTGACCG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Dynastes tityus was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid