Neoscona crucifera (Orb-weaver Spider)

December 28, 2025

Sample information
Picture
Entry by: Jack G
Location
Collection date 09/07/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

The spider was collected from a web in a pile of yard waste in a field.
Putative identification Arthropoda Arachnida Araneae Araneidae Neoscona Neoscona crucifera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes
  • Wear gloves to reduce risk of contamination.
  • Change pipette tips when pipetting different liquids.
  • Make sure to not pipette any of the pellet.
  • Make sure not to touch the pipette tip to the membrane. of the DNeasy column.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was a faint line in the Wolbachia lane of the gel; however, I deemed it to be due to cross-contamination, given its aforementioned faintness.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
ATATTAGGAACAGCAATAAGAGTATTAATTCGAATTGAGTTAGGTCAACCGGGTAGATTTATAGGTGATGACCAATTATATAATGTAATTGTAACTGCTCATGCATTTGTAATAATTTTTTTTATGGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTTCCACGAATGAATAATTTAAGATTTTGATTATTACCTCCTTCATTATTATTATTAATTACTTCATCAATAGTTGAAATAGGGGTTGGTGCTGGTTGAACAGTATATCCTCCTTTGGCTGGATTAGAAGGACATGCTGGTAGCTCAGTTGATTTTGCTATTTTTTCTTTACATTTAGCTGGGGCTTCTTCAATTATAGGGGCAATTAATTTTATTTCTACTATTATTAATATACGTTTTTATGGAATGACTATAGAAAAAGTTCCTTTATTTGTATGATCTGTATTAATTACAGCTGTTCTATTATTATTATCTTTACCANTATTANNTGGGGCTATTACAATATTATTAACTGATCAAAATTTTAATACTTCTTTTTTTGATCCTTCTGGGGGTGGAGATCCTATTTTATTTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Neoscona crucifera was found to be negative for Wolbachia.
Report Inappropriate Post