Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Dusty area, near bike racks, on the ground. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Camponotus |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Because I strictly followed my procedure and got results in the gels for my wolbachia and arthropod |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
TATTTGCTNTTTGAGCAGGAATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGAGGAGGTGATCCAATTCTATATCAACATTTATTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Camponotus was found to be postive for Wolbachia. |
European Paper Wasp
Woodworm Ant