Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Averaged-sized adult Yellowjacket, found on a hot steel sign outside on the lawn. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Qiagen DNeasy Blood and Tissue Kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | Removed abdomen from Yellow Jacket, crushed up and ran a test. Lane 2 is the Arthropod DNA, and Lane 3 is the Wolbachia DNA. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked, and there was no florecent line of any sort in lane 3. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
European Paper Wasp
Woodworm Ant